Influence of Calendula officinalis L. extract on the development of experimental hepatitis

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

The purpose of the study was to evaluate the effect of dry extract of Calendula officinalis L. on the course of experimental hepatitis caused by the administration of carbon tetrachloride in male Wistar rats.

Material and methods. The experimental hepatitis in Wistar rats was caused by four subcutaneous injections of a 50% oil solution of carbon tetrachloride at a dose of 4 ml/kg. The dry extract C. officinalis was administered intragastrically at a dose of 100 mg/kg to animals from the second day of the experiment for 20 days. The choleretic function of the liver was assessed in Wistar rats on 7th, 14th and 21th day. The intensity of oxidative stress, the state of the antioxidant system, the energy metabolism of hepatocytes and pathomorphological studies of the liver were held on 14th day.

Results. It has been established that C. officinalis dry extract in an experimental therapeutic dose has a beneficial effect on the course of acute toxic hepatitis in rats, reduced the intensity of free radical oxidation, limited the inflammatory reaction, reduced the severity of the main manifestations of liver damage – cytolysis, cholestasis, and also corrected energy processes in hepatocytes. Pathomorphological studies confirmed the hepatoprotective effect of the extract C. officinalis.

Conclusion. The dry extract C. officinalis at a dose of 100 mg/kg has a hepatoprotective effect in experimental toxic hepatitis.

全文:

受限制的访问

作者简介

А. Toropova

Institute of General and Experimental Biology

编辑信件的主要联系方式.
Email: anyuta-tor@mail.ru
ORCID iD: 0000-0003-2618-7777

Ph.D. (Biol.), Senior Research Scientist of Laboratory of Bioactive Compounds Safety

俄罗斯联邦, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

Z. Sambueva

Institute of General and Experimental Biology

Email: zinasamb@yandex.ru
ORCID iD: 0009-0009-2155-5789

Ph.D. (Biol.), Senior Research Scientist of Laboratory of Bioactive Compounds Safety

俄罗斯联邦, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

Ya. Razuvaeva

Institute of General and Experimental Biology

Email: tatur75@mail.ru
ORCID iD: 0000-0001-7829-1424

Dr. (Biol.), Senior Research Scientist of Laboratory of Bioactive Compounds Safety

俄罗斯联邦, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

D. Olennikov

Institute of General and Experimental Biology

Email: olennikovdn@mail.ru
ORCID iD: 0000-0001-8194-1061

Dr. (Pharm.), Head of the Laboratory of Biomedical Research

留尼汪, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

N. Badmaev

Institute of General and Experimental Biology

Email: nikita.bad@list.ru
ORCID iD: 0009-0009-9026-0992

Post-graduate Student of Laboratory of Experimental Pharmacology

俄罗斯联邦, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

S. Nikolaev

Institute of General and Experimental Biology

Email: smnikolaev@mail.ru
ORCID iD: 0000-0002-5833-7576

Dr. (Med), Professor, Chief Researcher of Laboratory of Experimental Pharmacology

俄罗斯联邦, 670047, Ulan-Ude, Sakh’yanovoy Str., 6

V. Khitricheyev

Buryat State University

Email: khitrikheev-ve@yandex.ru
ORCID iD: 0000-0002-4400-8085

Dr. (Med), Professor, Head of the Department of Hospital Surgery Medical Institute

俄罗斯联邦, 670000, Ulan-Ude, Smolina Str., 24A

参考

  1. Mancak M., Altintas D., Balaban Y. et al. Evidence-based herbal treatments in liver diseases. Hepatology Forum. 2024; 5(1): 50–60. doi: 10.14744/hf.2022.2022.0052.
  2. Hong M., Li S., Tan H.Y. et al. Current Status of Herbal Medicines in Chronic Liver Disease Therapy: The Biological Effects, Molecular Targets and Future Prospects. International Journal of Molecular Sciences. 2015; 16(12): 28705–28745. doi: 10.3390/ijms161226126.
  3. Колхир В.К., Багинская А.И., Сокольская Т.А и др. Разработка гастро- и гепатопротекторных средств на основе лекарственного растительного сырья. Опыт ВИЛАР. Вопросы биологической, медицинской и фармацевтической химии. 2013; 11: 41–48. [Kolkhir V.K., Baginskaya A.I., Sokolskaya T.A i dr. Development of gastro- and hepatoprotective agents based on medicinal plant raw materials. VILAR experience. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2013; 11: 41–48. (In Russ.)].
  4. Баторова С.М., Яковлев Г.П., Асеева Т.А. Справочник лекарственных растений традиционной тибетской медицины. Новосибирск: Наука, 2013; 292 с. [Batorova S.M., Yakovlev G.P., Aseeva T.A. Spravochnik lekarstvennykh rastenii traditsionnoi tibetskoi meditsiny. Novosibirsk: Nauka, 2013; 292 s. (In Russ.)].
  5. Colombo E., Sangiovanni E., D'Ambrosio M. et al. A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells. Evidence-Based Complementary and Alternative Medicine. 2015; 2015: 727342. doi: 10.1155/2015/727342.
  6. Fontes G.G., Freitas R.B., Almeida P.P. et al. Effects of Calendula officinalis extract on liver histopathology, lipid profile, and oxidative stress in rats submitted to a diet rich in cholesterol and carbohydrates. ACTA Cirúrgica Brasileira. 2023; 38: e383723. doi: 10.1590/acb383723.
  7. Кащенко Н.И. Фитохимическое исследование и совершенствование методов стандартизации цветков и травы календулы лекарственной (Calendula officinalis L.): Автореф. дис. канд. фарм. наук. Улан- Удэ, 2014; 22 с. [Kashchenko N.I. Fitokhimicheskoe issledovanie i sovershenstvovanie metodov standartizatsii tsvetkov i travy kalenduly lekarstvennoi (Calendula officinalis L.): Avtoref. dis. kand. farm. nauk. Ulan- Ude, 2014; 22 s. (In Russ.)].
  8. Olennikov D.N., Kashchenko N.I. New Isorhamnetin Glicosides and other Phenolic Compounds from Calendula officinalis. Chemistry of Natural Compounds. 2013; 49: 833–840. doi: 10.1007/s10600-013-0759-x.
  9. Руководство по проведению доклинических исследований лекарственных средств. Ч 1. Под ред. А.Н. Миронова. М.: Гриф и К, 2012; 944 с. [Rukovodstvo po provedeniyu doklinicheskikh issledovanii lekarstvennykh sredstv. Ch. 1. Pod red. A.N. Mironova. M.: Grif i K, 2012; 944 s. (In Russ.)].
  10. Мирошниченко В.П., Громашевская Л.Л., Касаткина М.Г. и др. Определение содержания желчных кислот и холестерина в желчи. Лабораторное дело. 1978; 3: 149–158. [Miroshnichenko V.P., Gromashevskaya L.L., Kasatkina M.G. i dr. Determination of bile acids and cholesterol in bile. Laboratornoe delo. 1978; 3: 149–158. (In Russ.)].
  11. Patergnani S., Baldassari F., De Marchi E. et al. Methods to monitor and compare mitochondrial and glycolytic ATP production. Methods in Enzymology. 2014; 542: 313–332. doi: 10.1016/B978-0-12-416618-9.00016-9.
  12. Kikugawa K., Kojima T., Yamaki S. et al. Interpretation of the thiobarbituric acid reactivity of rat liver and brain homogenates in the presence of ferric ion and ethylenediaminetetraacetic acid. Analytical Biochemistry. 1992; 202: 249–255. doi: 10.1016/0003-2697(92)90102-D.
  13. Hamza T.A., Hadwan M.H. New spectrophotometric method for the assessment of catalase enzyme activity in biological tissues. Current Analytical Chemistry. 2020; 16: 1054–1062. doi: 10.2174/1573411016666200116091238.
  14. Pinto R.E., Bartley W. The effect of age and sex on glutathione reductase and glutation peroxidase activities and aerobic glutathione oxidation in rat liver homogenates. Biochemical Journal. 1969; 112(1): 109–115. doi: 10.1042/bj1120109.
  15. Shaik I.H., Mehvar R. Rapid determination of reduced and oxidized glutathione levels using a new thiol-masking reagent and the enzymatic recycling method: Application to the rat liver and bile samples. Analytical and Bioanalytical Chemistry. 2006; 385(1): 105 -113. doi: 10.1007/s00216-006-0375-8.
  16. Коржевский Д.Э., Гиляров Ф.В. Основы гистологической техники. Спб.: СпецЛит, 2010; 95 с. [Korzhevskii D.E, Gilyarov F.V. Osnovy gistologicheskoi tekhniki. Spb.: SpeTSLit, 2010; 95 s. (In Russ.)].
  17. Zhangabay Z., Berillo D. Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract. Results in Surfaces and Interfaces. 2023: 11: 100109. doi: 10.1016/j.rsurfi.2023.100109.
  18. Wan L., Jiang J.-G. Protective effects of plant-derived flavonoids on hepatic injury. Journal of Functional Foods. 2018: 44: 283–291. doi: 10.1016/j.jff.2018.03.015.
  19. Tan P., Jin L., Qin X. et al. Natural flavonoids: Potential therapeutic strategies for non-alcoholic fatty liver disease. Frontiers in Pharmacology. 2022; 13: 1005312. doi: 10.3389/fphar.2022.1005312.

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Effect of C. officinalis extract on the state of pro- and antioxidant systems in the liver in Wistar rats with CCl4-hepatitis (14th day): GPO – glutathione peroxidase, RG – reduced glutathione, GR – glutathione reductase, MDA – malondialdehyde

下载 (102KB)
索引源数据
3. Fig. 2. The effect of C. officinalis extract on the energy status of the liver in Wistar rats with CCl4-hepatitis (14th day): MK – lactic acid, PVC – pyruvic acid, ATP – adenosine triphosphoric acid

下载 (90KB)
索引源数据

版权所有 © Russkiy Vrach Publishing House, 2024