Development of approaches to standardization of fresh fruits of Crataegus submollis Sarg

Actuality. The fruits of Crataegus submollis Sarg. (family Rosaceae) are a popular food product, but are not currently used in medical practice. Previously, the possibility of using of the fresh fruits of Crataegus submollis Sarg. as a source of antidepressant and diuretic drugs has been shown. One of the new drugs may be a juice based on fresh fruits of Crataegus submollis Sarg. In еру fresh fruits of the Crataegus submollis Sarg. predominate the reduced forms of flavonoids, namely procyanidins. An earlier analysis of the juice from fresh fruits of Crataegus submollis Sarg. with the using of spectrophotometry showed that the maximum absorption in the UV spectrum is 282 nm, which coincides with the maximum absorption of the solution of catechin, a biogenetic precursor of procyanidins. The aim is the development of the method for quantitative determination of the total procyanidins in of the fresh fruits of Crataegus submollis Sarg. Material and methods. The object of the study was fresh fruits of Crataegus submollis Sarg. harvested in September 2019 on the territory of Samara and Samara region. The method of analysis was direct spectrophotometry at a wavelength of 282 nm calculated on catechin. Results. As a results of the study the method of quantitative determination of the total procyanidins (calculated on catechin) in fresh fruits of Crataegus submollis Sarg. by direct spectrophotometry at an analytical wavelength of 282 nm was developed. It was determined that the content of the total procyanidins in fresh fruits of Crataegus submollis Sarg. varies from 0,52±0,03% to 0,97±0,06% (calculated on catechin). The error of a single determination with a 95% confidence probability is ±5,81 %. Conclusion. The highest content of procyanidins can be noted in the sepals and the skin of еру fresh fruits of Crataegus submollis Sarg. The fresh fruits of Crataegus submollis Sarg. are promising medicinal plant raw materials.

Crataegus submollis Sarg, Crataegus submollis Sarg, fruits, flavonoids, procyanidins, catechin, spectrophotometry