Development and validation of a spectrophotometric method for the quantitative analysis of the substance of a new biologically active compound 3-[2-oxo-2-(4-phenyl-1-piperazinyl)ethyl]-4(3H)-quinazolinone

The purpose of the work is to study the possibility of using spectrophotometry in the UV region for the quantitative determination of a previously unstudied substance of 3- [2- (4-phenyl-1-piperazino) -2-oxoethyl] -quinazolin-4 (3H) -one (VMA-10 -21), as well as the development and validation of methods for its quantitative determination. Material and methods. In the course of the experiment, the analytical equipment was used: spectrophotometer SF-56, analytical balance RADWAG AS 220/C/2, measuring dishes of the 1st accuracy class. The optical density of the test solutions was measured at a wavelength of 275 nm in cuvettes with a layer thickness of 10 mm. As a comparison solution used 1% solution of hydrochloric acid. Validation of the presented methodology was carried out according to indicators: linearity, precision and correctness. Processing of the experimental data was carried out according to OFS.1.1.0013.15 GF XIV edition. The specific absorption index was calculated using a standard sample of substance VMA-10-21, the purity of which was proved using the HPLC method. Results. The spectrum of the substance VMA-10-21 in the ultraviolet region was represented by two absorption bands with maxima at wavelengths of 235 and 275 nm. The use of a wavelength at a wavelength of 275 nm is justified. The specific absorption index at the selected wavelength was calculated. A quantitative determination technique has been developed which, according to validation parameters, linearity, precision and correctness complies with the requirements of OFS.1.1.0012.15 GF of the XIV edition. Conclusions. The possibility of using spectrophotometry in the UV region for the quantitative determination of the substance VMA-10- 21 was studied. A spectrophotometric method for the quantitative determination of the basic substance in the substance VMA-10-21 was developed and validated.

spectrophotometry, quinazoline derivative, quantification, validation