Vol 23, No 4 (2020)

The effect of foliar treatment of plants with a phytoindesaturase herbicide inhibitor on the content and composition of the essential oil (EM) of Elsholtzia ciliata (Thunb.) Hyl. It has been established that, depending on the concentration of the drug, the hormonal balance in the redox enzymatic complex changes, which is manifested in a change in the composition of the EM, in particular, in the content of the main components: ketone elsholtion and ketone dehydroelsoltion. Processing plants E. ciliata (Thunb.) Hyl. drug diflufenikan causes a change in the hormonal balance in the multienzyme complex of the biosynthesis of essential oils, mainly affecting the balance of redox enzymatic systems. The intensity and nature of the effect depends on the concentration of the active substance in the solution: when the concentration of diflufenicane is 0.05 g/l, the inhibitory effect of the drug on the processes of biosynthesis of essential oils is observed. Treatment with the drug in lower concentrations of 0.005 and 0.0005 g/l leads to a shift in the equilibrium of the redox enzyme system towards the activation of dehydrogenases and an increase in the amount of ketone in the essential oil. Pre-harvesting plants with E. ciliata (Thunb.) Hyl. diflufenikan allows you to purposefully influence the change in the content of the main components of essential oils in the direction of the formation of more unsaturated components, which increases the antioxidant properties of the oil and its antimicrobial and antiviral activity.

Relevance. The use of non-steroidal anti-inflammatory drugs (NSAIDs) is not limited to medical indications. Drugs are often used to relieve pain in drug addiction, so they are often found during forensic analysis along with psychoactive substances. The identification and measurement of the concentration of any toxicants is important for establishing a causal relationship between their use and harm to health or death. Purposes of the work: to identify the use of NSAIDs by different groups of consumers; determination of the possibility of using these drugs as indirect markers of the use of controlled substances; establishing a correlation between the joint detection of NSAIDs and controlled substances; comparison of detected concentrations of substances with therapeutic, toxic, lethal concentrations. The research material was the results of the work of the forensic chemical department of the Krasnoyarsk Bureau of Forensic Medical Examination for 2016-2018. Biological material research methods included liquid-liquid extraction of compounds from objects, gas chromatography with mass spectral detection after derivatization. The results of forensic chemical studies were processed and interpreted using factor, regression, and correlation analysis methods. Results. Data were obtained on the age-sex characteristics of NSAIDs consumers, a positive correlation between the use of NSAIDs and controlled substances, the range of concentrations of NSAIDs in cadaveric blood was determined. Conclusions. An increase in the detection of NSAIDs during forensic chemical studies was revealed. The main consumers of this group of medicines are persons aged 31 to 45 years; the number of male consumers prevails over women. Detection of NSAIDs in biological fluids of cadavers and a strong positive correlation may serve as markers of possible intravital use of controlled substances. Excess toxic concentrations of NSAIDs in cadaveric blood was recorded in 0.33% of cases, typical only for ibuprofen and ketorolac.Key words: forensic chemical analysis, non-steroidal anti-inflammatory drugs, controlled substances, use marker, correlation.

In modern society, necessity for neuroprotectors is increasing, including drugs with nootropic and antioxidant effects. Chemical screening of nootropic species showed the presence of various groups of biologically active substances. Phenolic compounds are dominant, including flavonoids, which are known for their antioxidative activity. The aim. To conduct a study of the qualitative composition and quantitative content of flavonoids and determine the antioxidant activity of the developed nootropic species. Material and methods. Qualitative analysis was carried out by TLC. The quantitative content of flavonoids was determined by spectrophotometric method. The antioxidant activity of nootropic species was proved by spectrophotometric method based on the inhibition reaction of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). Results. As a result of the research, the qualitative composition of the nootropic species was studied. Up to 9 phenolic compounds were found, among them the following compounds were identified: rutin, quercetin, hyperoside, and chorogenic acid. The sum of flavonoids in terms of rutin in the nootropic species was 2.22 ± 0.11%. As a result of the research, the antioxidant activity of nootropic species was proved, the IC50 value was 6.33 ± 0.25 mg / ml. Conclusion. As a result of the study, the qualitative composition of the nootropic species was examined and the sum of flavonoids in terms of rutin was determined. The antioxidant activity of nootropic species has been proven.

In the course of preclinical testing of the substance, research is needed aimed at developing methods of analysis, standardization and the creation of a regulatory document - a pharmacopeia article (FS). To assess the quality of pharmaceutical substances, various quantitative methods are used (titrimetric, chromatographic, spectral). Many methods use standard samples, which are very difficult to obtain when developing methods of a new biologically active substance. The titrimetric methods of pharmaceutical analysis are quite accurate, affordable, simple and do not require complex and expensive equipment and reagents. The purpose of this study is the development and validation of a method for the quantitative determination of the investigated substance - 2-benzoylamino-N- [4-(4,6-dimethylpyrimidin-2-ylsulfamoyl) phenyl] benzamide. During pharmacological studies, anxiolytic, actoprotective and antidepressant activities were established for this compound. Material and methods. The objects of study were the studied samples of the substance 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl) phenyl] benzamide synthesized at the Department of Organic Chemistry of the Pyatigorsk Medical and Pharmaceutical Institute. Results. The results of the developed procedure for the quantitative determination by a non-aqueous titration method of a new quinosalinone derivative of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl) phenyl] benzamide are presented. It has been shown that the solvent system is the most protogenic medium for the substance under study. glacial acetic acid - formic acid 10:10 ml. When developing the method using the crystalline violet indicator, the average content of the test substance is 97.12%, the relative error is 2.21%. The potentiometric determination of the end point of the titration showed that the average content of the test substance is 98.67%, the relative error is 1.57%. Conclusion. Thus, we have developed a method for the quantitative determination of a new quinosalinone derivative with pharmacological activity - 2-benzoylamino-N- [4- (4,6-dimethylpyrimidin-2-ylsulfamoyl) phenyl] benzamide. The end point of the titration is determined along with the classic indicator crystal violet, it is proposed to carry out using the potentiometric method. A validation assessment of the developed methodology was carried out according to the indicators: linearity, convergence and correctness.