Development and validation of a method for the quantitative determination of 20-hydroxyecdisone in the serratula coronata herbal dry extract

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Introduction. The development and validation of analytical methods are one of the most important stages in the development of a new drug. As part of the implementation of the scientific research plan, the VILAR has developed a method for obtaining a dry extract Serratula coronata herb and its adaptogenic activity has been proven.

The purpose of the study was to develop and validate a method for the quantitative determination of 20-hydroxyecdysone to control the quality of the Serratula coronata herbal dry extract.

Materials and methods. The object of the study is a dry extract obtained by extracting dried herb with 70% ethyl alcohol, subsequent concentration, purification with chloroform, liquid-liquid extraction with n-butanol and drying of butanol extracts.

Results. As a result of the research, optimal analysis conditions were selected and validation was carried out, which made it possible to characterize it positively in terms of specificity, linearity, accuracy, reproducibility, and intra-laboratory precision.

Conclusions. The developed method is suitable for the quantitative determination of 20-hydroxyecdysone in the Serratula coronata herbal dry extract and can be used in drawing up a regulatory document for this substance.

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作者简介

G. Adamov

Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR)

编辑信件的主要联系方式.
Email: grig.adamov@mail.ru
ORCID iD: 0000-0001-7347-175X

Ph.D. (Pharm.), Laboratory of Atomic-Molecular Bioregulation and Selection

俄罗斯联邦, Grina str. 7, Moscow, 117216

A. Radimich

Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR)

Email: vilarnii.radimich@mail.ru
ORCID iD: 0000-0002-1139-8902

Senior Research Scientist, Department of Chemistry of Natural Compounds

俄罗斯联邦, Grina str. 7, Moscow, 117216

O. Saybel

Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR)

Email: olster@mail.ru
ORCID iD: 0000-0001-8059-5064

Dr.Sc. (Pharm.), Head of the Center for Chemistry and Pharmaceutical Technology

俄罗斯联邦, Grina str. 7, Moscow, 117216

参考

  1. Bathori M., Toth N., Hunyadi A., Marki A., Zador E. Phytoecdysteroids and anabolicandrogenic steroids: structure and effects on humans. Curr. Med. Chem. 2008; 15: 75–91.
  2. Khaziev D., Galina C., Gadiev R., et al. Phytoecdisteroids from Serratula coronata when growing ducklings. Res. Vet. Sci. 2020; 128: 170–176. doi: 10.1016/j.rvsc.2019.11.012.
  3. Catalan K.D., Martinez A.M., Aragones M.D., et al. Alterations in rat lipid metabolism following ecdysterone treatment. Comp. Biochem. Physiol. (B). 1985; 81: 771–775.
  4. Gao L., Cai G., Shi X. b-Ecdysterone induces osteogenic differentiation in mouse mesenchymal stem cells and relieves osteoporosis. Biol. Pharm. Bull. 2008; 31: 2245–2249.
  5. Tang Y.H., Yue Z.S., LiG. S., et al. Effect of β-ecdysterone on glucocorticoid-induced apoptosis and autophagy in osteoblasts. Mol. Med. Rep. 2018; 17(1): 158–164. doi: 10.3892/mmr.2017.7840.
  6. Zhang X., Xu X., Xu T., Qin S. β-Ecdysterone suppresses interleukin-1β-induced apoptosis and inflammation in rat chondrocytes via inhibition of NF-κB signaling pathway. Drug Dev. Res. 2014; 75(3): 195–201. doi: 10.1002/ddr.21170.
  7. Punegov V. V., Sychov R. L., Zainullin V. G., et al. Extraction of ecdysteron-80 from Serratula coronatа L. and assessment of its pharmacological action. Part I. Adaptogenic, gastroprotective, thermoprotective, and antihypoxic activity. Pharmaceutical Chemistry Journal. 2008; 42: 446–451. doi: 10.1007/s11094-008-0159-x.
  8. Volodin V.V. Fitojekdisteroidy. SPb.: Nauka. 2003. 293 s.
  9. Dinan L. The Karlson Lecture. Phytoecdysteroids: What use are they? Arch. Insect. Biochem. Physiol. 2008; 72: 126–141. doi: 10.1002/arch.20334.
  10. Bathori M., Pongracz Z. Phytoecdysteroids – from isolation to their effects on humans. Current Medicinal Chemistry. 2005; 12: 153–172.
  11. Olennikov D.N., Kashhenko N.I. Fitojekdisteroidy rodov Serratula L. i Klasea Cass. (Asteraceae): hemoraznoobrazie, metody vydelenija i analiza. Himija rastitel'nogo syr'ja. 2017; 4: 123–135.
  12. Báthori M., Zupkó I., Hunyadi A., et al. Monitoring the antioxidant activity of extracts originated from various Serratula species and isolation of flavonoids from Ser-ratula coronate. Fitoterapia. 2004; 75(2): 162–167. doi: 10.1016/j.fitote.2003.12.009.
  13. Angaskieva A.S., Andreeva V.Ju., Kalinkina G.I., et al. Issledovanie himicheskogo sostava serpuhi vencenosnoj, kul'tiviruemoj v Sibiri. Himija rastitel'nogo syr'ja. 2003; 4: 47–50.
  14. Mjagchilov A.V. Flavonoidy rastenij grechihi posevnoj (Fagopyrum sagittatum Gilib.) i serpuhi vencenosnoj (Serratula coronata L.) (metody vydelenija, identifikacija veshhestv, perspektivy ispol'zovanija): Avtoref. diss. … kand. Biol. nauk. Vladivostok. 2015. S. 21.
  15. Mjagchilov A. V., Sokolova L. I., Gorovoj P. G. Novye flavonoidy rastenija Serratula coronata L. Himiko-farmacevticheskij zhurnal. 2017; 51(2): 23–27.
  16. Mjagchilov A. V., Sokolova L. I., Gorovoj P.G. 3-metil-kvercetin-4’-O-β-D-gljukuronopiranozid – novyj flavonoid, vydelennyj iz Serratula coronata L. Himija ras-titel'nogo syr'ja. 2016; 4: 77–81.
  17. Angaskieva A.S. Farmakognosticheskoe issledovanie serpuhi vencenosnoj, kul'tiviruemoj v Sibiri: Avtoref. diss. … kand. Farm. nauk. Tomsk. 2006. S. 19.
  18. Lupanova I.A., Sajbel' O.L., Ferubko E.V. i dr. Farmakologicheskij skrining jekstrakta travy serpuhi vencenosnoj (Serratula coronata L.). Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2022; 25(3): 43–48. doi: 10.29296/25877313-2022-03-06.
  19. MZ R. F. M. Gosudarstvennaja farmakopeja Rossijskoj Federacii. XIV izd. OFS 1.1.0013.15 Statisticheskaja obrabotka rezul'tatov himicheskogo jeksperimenta. T. 1. 2018. S. 289.
  20. MZ R. F. M. Gosudarstvennaja farmakopeja Rossijskoj Federacii. XIV izd. OFS 1.1.0012.15 Validacija analiticheskih metodik. T. 1. 2018. S. 276.
  21. Bagirova V.L. i dr. Rukovodstvo dlja predprijatij farmacevticheskoj promyshlennosti. M.: Sport i kul'tura. 2007.

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2. Fig. 1. HPLC chromatogram of sample solvent, test solution, standard solution, column 1, y-axis – optical density, x-axis – time, min

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3. Fig. 2. UV chromatograms of 20-hydroxyecdysone on chromatograms of the standard (1) and test (2) solution at different wavelengths

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