Email this article Mechanical preparation of the amniotic membrane in the creation of bioengineered structures for the restoration of corneal epithelium
- Authors: Gavrilyuk I.O.1, Aleksandrova O.I.2, Kuznetsova A.Y.1, Mashel T.V.2,3, Seleznev A.S.1, Chernysh V.F.1, Churashov S.V.1, Blinova M.I.2, Kulikov A.N.1
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Affiliations:
- Kirov Military medical academy
- Institute of Cytology of the Russian Academy of Sciences
- Peter the Great St.Petersburg Polytechnic University
- Issue: Vol 21, No 4 (2019)
- Pages: 116-120
- Section: Articles
- URL: https://journals.eco-vector.com/1682-7392/article/view/20688
- DOI: https://doi.org/10.17816/brmma20688
- ID: 20688
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Full Text
Abstract
The «gold standard» among biological and synthetic scaffolds for cultivation is the amniotic membrane. Its preparation for the needs of tissue engineering is associated with the difficulties of transporting and preserving the native amniotic membrane. The amniotic membrane was taken after elective caesarean section. The separated amniotic membrane was fixed according to our method [5]. Scaffolds were divided into 3 groups of 5 membranes each: storage under hypothermia, cryopreservation at –20 °C and –80 °C. Stem cells of the corneal epithelium of rabbits were used as a test system, and cells cultured under standard conditions were used as a control. Viability was determined using phase contrast microscopy and microtiter test. It has been suggested that the inhibition of the state of cells cultured on the amniotic membrane by the 14th day is associated with the viability of the own cells of the amniotic membrane. To verify this assumption, a microtiter test was carried out for all scaffold groups. The described method of immobilization of the amniotic membrane provides transportation, preservation and the possibility of culturing stem cells on the amniotic membrane. For the cultivation of stem cells during the first day, all three types of preservation of the amniotic membrane are suitable. In order to create bioengineered structures for restoration of the corneal epithelium, further research is needed to find the optimal way to de-epithelialize the amniotic membrane.
About the authors
I. O. Gavrilyuk
Kirov Military medical academy
Author for correspondence.
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg
O. I. Aleksandrova
Institute of Cytology of the Russian Academy of Sciences
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg
A. Yu. Kuznetsova
Kirov Military medical academy
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg
T. V. Mashel
Institute of Cytology of the Russian Academy of Sciences; Peter the Great St.Petersburg Polytechnic University
Email: vmeda-nio@mil.ru
Russian Federation, Санкт-Петербург; Санкт-Петербург
A. S. Seleznev
Kirov Military medical academy
Email: vmeda-nio@mil.ru
Russian Federation, Санкт-Петербург
V. F. Chernysh
Kirov Military medical academy
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg
S. V. Churashov
Kirov Military medical academy
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg
M. I. Blinova
Institute of Cytology of the Russian Academy of Sciences
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg
A. N. Kulikov
Kirov Military medical academy
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg
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