基于动物主动脉弹性蛋白的异种生物材料应用于组织重塑中的可行性论证

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详细

本研究试图解决实验和再生医学中的一个课题，即通过完全脱细胞化，从驯鹿主动脉中提取无抗原性的原生弹性蛋白，创造一种新的生物材料，目的是将其用作损伤后组织重塑的生物材料。研究治疗热烧伤和创伤（包括枪伤）的新方法和用品是一项紧迫任务，特别是考虑到当前的现实情况（大规模军事行动）。针对在热伤和枪伤（包括穿透伤）的加速修复过程时，完善治疗方法的必要性，以及并发症和永久性残疾的高风险，产生从驯鹿结缔组织中提取降低抗原性的生物可吸收材料的想法。纤维状弹性蛋白是众多器官中结缔组织不可分割的一部分，它负责拉伸。在本研究中，选择弹性动脉，即驯鹿主动脉，作为弹性蛋白的来源。通过机械、化学和生化处理，去除具有抗原特性的细胞、胶原纤维和间质，获得基于弹性蛋白的生物材料。使用光镜和电子显微镜对生物材料进行了分析。 获得的生物材料被用作异种移植，不显示抗原特性，可在热损伤后恢复组织完整性。已确定，按照所开发的技术使用该生物材料时，在实验鼠身上未观察到排异反应，这证明了所获得的生物材料没有免疫原性。这表明所获得的生物材料有可能应用于主动脉的组织重塑，用来加速这一过程。

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作者简介

Nikita A. Shutskiy

Northern State Medical University; Northern (Arctic) Federal University named after M.V. Lomonosov

编辑信件的主要联系方式.
Email: nikitashutskijj@rambler.ru
ORCID iD: 0000-0003-0979-1569
SPIN 代码: 1430-4970

Cand. Sci. (Biology)

俄罗斯联邦, Arkhangelsk; Arkhangelsk

Sergey L. Kashutin

Northern State Medical University

Email: sergeycash@yandex.ru
ORCID iD: 0000-0002-2687-3059
SPIN 代码: 7623-7216

MD, Dr. Sci. (Medicine), associate professor

俄罗斯联邦, Arkhangelsk

Lyubov N. Gorbatova

Northern State Medical University

Email: info@nsmu.ru
ORCID iD: 0000-0003-0675-3647
SPIN 代码: 8037-5341

MD, Dr. Sci. (Medicine), professor

俄罗斯联邦, Arkhangelsk

Nikita S. Kholopov

Northern State Medical University

Email: nikitaholopov@mail.ru
ORCID iD: 0000-0001-5604-5257
SPIN 代码: 6776-4068

postgraduate

俄罗斯联邦, Arkhangelsk

D. V. Mizgirev

Northern State Medical University

Email: denimsur@rambler.ru
ORCID iD: 0000-0002-6804-3790
SPIN 代码: 6202-6807

MD, Dr. Sci. (Medicine), associate professor

俄罗斯联邦, Arkhangelsk

A. K. Sherstennikova

Northern State Medical University

Email: a.sherstennikova@yandex.ru
ORCID iD: 0000-0001-8576-6459
SPIN 代码: 7473-3278

MD, Dr. Sci. (Medicine), associate professor

俄罗斯联邦, Arkhangelsk

Nikolay V. Sherstennikov

Northern State Medical University

Email: shersten96@gmail.com
ORCID iD: 0009-0004-1606-3775
SPIN 代码: 2684-8233

junior research assistant

俄罗斯联邦, Arkhangelsk

Leonid L. Shagrov

Northern State Medical University

Email: leonidshagrov@mail.ru
ORCID iD: 0000-0003-2655-9649
SPIN 代码: 3842-2145

junior research associate

俄罗斯联邦, Arkhangelsk

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2. Fig. 1. Aortic fragment after treatment in 20% sodium dodecyl sulfate solution

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3. Fig. 2. Photomicrographs of aorta fragment sections stained with hematoxylin/eosin obtained before (a) and after treatment with detergent (b). Magnification: ×10 objective, ×40 ocular

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4. Fig. 3. Photomicrographs of aorta fragment sections stained using the Van Gieson method obtained before (a) and after treatment with detergent (b). Magnification: ×10 objective, ×40 ocular

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5. Fig. 4. Photomicrographs of aorta fragment sections stained with hematoxylin/eosin obtained before (a) and after treatment with detergent (b). Magnification: ×10 objective, ×10 ocular

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6. Fig. 5. Scanning electron microscopy photomicrographs of aorta fragment specimens before treatment with detergent. Magnification: ×500

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7. Fig. 6. Scanning electron microscopy photomicrographs of aorta fragment specimens after treatment with detergent. Magnification: ×500

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8. Fig. 7. Photomicrographs of aorta fragment sections stained using the Feulgen method obtained before (a) and after treatment with detergent (b). Magnification: ×40 objective, ×40 ocular

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9. Fig. 8. Rats with xenogenic aorta-derived biomaterial obtained using the proposed technology: a — Day 3 of the experiment; b — Day 21 of the experiment

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