Vol 2, No 5 (2014)

We have studied carboxylic acids of the herb of Thymus cretaceus Klok. et Schost which is widespread on a territory of some regions (Belgorod, Voronezh). The study was carried out using gas-liquid chromatography at Agilent Technologies 6890 chromatographer with mass-spectrometric detector 5973 N. Acids concentration was calculated by means of inner standard. We have established that carboxylic acids of Thymus cretaceus are represented by 34 compounds. Palmitic (1779.02 mg/kg), behenic (1084.15 mg/kg), levulinic (986.24 mg/kg) and linoleic acids (678.82 mg/kg) predominate among fatty acids; citric (9835.14 mg/kg), malonic (447.91 mg/kg) and oxalic acids (388.32 mg/kg) predominate among organic acids; andferulic acid predominate amongphenolcarbonic acids.

We have studied conditions for metaprote extraction from water solutions with pH indices 1 - 12.45. We have also established that metaprote is maximally extracted with chloroform with pH index 6.86 - 9.19, with chloroform-acetone mixture 9:1 with pH index 4.01 - 12.45. We have determined the probability of UV spectrophotometry application for detection and identification of metaprote in extracts. The results received are recommended for use in development of method of metaprote isolation from biological liquids and its detection and identification with UV spectrophotometry for chemical and toxicological analysis.

Hepatic decompensation problems make it timely to search for the methods of its treatment. Stem cells usage in attempt to restore structures of organs and tissues is a promising direction of researches. However the problem of possible blast-cell transformation slows down studies in this direction. Attempt of thymalinum use as an antitumoral immune system's modulator may be successful and may widen the possibilities of stem cells use in hepatology. On the basis of toxical affection of rats' lever by tetrachloromethane and paracetamol we have studied hepatoprotective activity of thymalinum and suspension of rats' red bone marrow (RBM) and a thymalinum + suspension of RBM complex. Hepatoprotective action was estimated by the volume of discharged bile of control group rats which received paracetamol comparing with intact animals. This confirms the absence of reliable hepatotoxical action of paracetamol following the methodology applied. Significant reduction of discharged bile volume of control group rats which received tetrachloromethane comparing with intact animals confirms the successfulness of the formation method of hepatitis model in animals which received tetrachloromethane. The animals which were given tetrachloromethane and thymalinum + suspension of RBM combination had bigger volume of bile discharged than control group animals. Hepatoprotective action tendency of thymalinum + suspension of RBM combination shown before on mice is also true for rats.

The article presents the results of an opinion poll of pharmacies visitors in Odessa, Ukraine about understanding and interpretation of the instructions for medical use of drugs. Results of the study identified a particular patients’ perception of the semantic content of the instructions texts and the need for further information and methodological work to improve their communicative accuracy.

The article presents studies on development of cosmetics with complex extracts from herb of Bidens, flowers of Calendula, and flowers of Matricaria. We cited the analysis methods of the received extract and a drug on its base.