Vol 12, No 3 (2014)

The review is devoted to the role of mitochondrial Ca2+-dependent pore (mPTP) in the regulation of metabolic processes in cells under physiological and pathological conditions. The mechanisms of reperfusion injury in the postischemic period involving mPTP are discussed in the paper. The possibilities of pharmacological regulation of metabolic and functional processes in cells by target action on mPTP work are assessed. This approach allows to regulate key cell functions, stimulating either mechanisms of adaptation and survival in extreme conditions or apoptosis. Pharmacological modulators of the mitochondrial pore as drugs have promising value for treatment of ischemic diseases as well as tumor therapy.

The hypolipidemic efficiency of antihypoxant hypoxen in the experimantal conditions of alimantary dislipoproteinemia (DLP) in rats and guinea pigs in comparison with lipid-lowering drugs gemfibrozyl and probucol in doclinic investigation was studied. The alimantary models of DLP were created by feeding of rats and guinea pigs of hypercholesterol diet (rich content of food cholesterol, the plant and animal lipids, vitamin D2) during 30 days. The concentration of lipid oxidative products in serum and indicators of lipid metabolism (content of TCh, TG, HDL-Ch) in blood and liver were analysed. The calculation of cholesterol index of atherogenity was used. It was shown that hypoxen in dose 50 mg/kg per os had been decreased the concentration of lipid oxidative products in serum and revealed the pronouced lipid-lowering effect in blood and liver of aninals. The hypolipidemic efficiency of hypoxen was in the proximity of gemfibrozyl and probucol. The experimantal results have been shown the possibility of practic using of this active antihypoxant. Hypoxen seemingly to be useful as a hypolipidemic drug in the complex therapy of cardiovascular and atherosclerosis associated diseaseis.

The purpose of the investigation was to clear the significance of dopamine, GABA, opioids and sodium influx ionic currents of the nucleus accumbens neurons for the reinforcing effects of a number of psychotropic drugs (opiates, opioids, psychostimulants) on conditioned place preference (CPP) in rats. The microcannules were implanted into the nucleus accumbens (the extended amygdala system) of the Wistar male rats to inject the drugs studied (1 μg in 1 μl in volume for each injection). The rats were learned CPP of a one of narcogenics during 8 days. Some drugs, lidocain, a blocker of sodium influx ionic currents, antagonists of GABAA receptors bicuculline, D1 dopamine receptors SCH23390, D2 dopamine receptors sulpiride and opioid receptors naloxone, administered intrastructurally into the nucleus accumbens, were used for pharmacological analysis. The majority of the blockers studied decreased or abolished the reinforcing effects of amphetamine. Activation of reinforcement by means of fentanyl was reversed with bicuculline, lidocain and naloxone but did not change with dopamine antagonists (SCH23390 and sulpiride). None of the blockers studied effect on CPP of sodium ethaminal excluding bicuculline which reduced it. At last, the leu-enkephaline effects were reversed with naloxone and SCH23390, but strengthened with bicuculline. Sulpiride and lidocain did not effect on CPP of leu-enkephaline. Therefore, the different mechanisms (GABA-, dopamine- and opioidergic) controlling the positive conditioned reinforcement are collected in the nucleus accumbens.

The action of anxiolytic afobazol (2-mercaptobenzimidazolum derivative) in concentration 1 mM and local anesthetic bupivacaine in concentrations 0,5-1 mM on ionic currents of isolated neurons of Lymnaea stagnalis and Planorbarius corneus was studied. The method of intracellular dialysis was used in the experiment. Afobazol and bupivacaine after intracellular administration was shown not to possess their inhibition effect of ionic currents, typical for extracellular administration of drugs. It is suggested that pharmacological targets or bindind sits for these drugs are located on external surface of neuronal membrane.

"The Canon of Medicine" has a thousand-year history to this day, remains its relevance. Data submitted by our studies, analyzed, taking into account the achievements of modern phytochemistry, phytopharmacology and medicine. These data are invaluable as a resource base for all groups of professionals in the field of medicine and health care and related industries and individuals, who make decisions on preservation of their own health.