Changes in the activity of lysosomal cysteine proteases in vascular wall homogenates in varicose veins of the lower extremities

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Abstract

Relevance. The article deals with the issue of changes in the activity of lysosomal cysteine proteases – cathepsins L, B, H in homogenates of vascular wall, obtained from patients with varicose veins of the lower extremities. Due to the fact that the proteolytic processes occurring in the wall of varicose transformed veins currently remain poorly understood, and the incidence of varicose veins is increasing, the relevance of the topic remains at a high level.

Materials and methods. The study shows the results from 22 patients divided into two groups: the first group (n=12): patients with varicose veins of the lower extremities, the second group (control) – patients who had healthy vein sections, taken during femoral-popliteal bypass surgery using autovena (n=10). The material for the study was homogenates of vascular wall, obtained during surgical intervention. The vessels were weighed on electronic scales (Ohaus corporation, USA). The samples were crushed and placed in a glass of the Potter S homogenizer (Sartorius, Germany). Homogenized for 60 seconds with a rotation speed of a Teflon pestle of 1500 rpm. The activity of cathepsins B, L and H was determined spectrofluorimetrically on the RF – Zhimadzu Spectrofluorometer (Japan). The protein content was assessed using the Lowry method by the commercial set of the NPC "Eco-service" (St. Petersburg).

Results. The activity of cathepsin B in the homogenate of varicose veins was increased by 35 times compared to the control group. The activity of cathepsin D in the homogenate of varicose veins was also increased 17-fold compared to the control. The activity of cathepsin H in varicose vein homogenate showed a unidirectional trend - it was higher than the control values, but by a smaller number of times – by 9. Thus, in varicose vein homogenates, all three studied cathepsins increase their activity, but to varying degrees. The greatest changes, according to our study, confirmed the activity of cathepsin B.

Conclusions. Varicose veins of the lower extremities are accompanied by a significant increase in the activity of lysosomal cysteine proteinases – cathepsins B, L and H in the wall homogenates of the affected vein, which may indicate the involvement of LC in the pathogenesis of venous transformation.

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About the authors

M. G. Konopleva

Ryazan State Medical University

Author for correspondence.
Email: mari.konopleva.97@mail.ru

Senior Laboratory Assistant-Researcher

Russian Federation, Ryazan

Z. S. Gazaryan

GBU RO OKB

Email: GhazaryanZ@yandex.ru

Orthopedic Traumatologist

Russian Federation, Ryazan

R. E. Kalinin

Ryazan State Medical University

Email: kalinin-re@yandex.ru

Dr.Sc. (Med.), Professor, Head of Department of Cardiovascular, X-Ray Endovascular Surgery and Radiation Diagnostics

Russian Federation, Ryazan

I. A. Suchkov

Ryazan State Medical University

Email: i.suchkov@rzgmu.ru

Dr.Sc. (Med.), Professor, Department of Cardiovascular, X-Ray Endovascular Surgery and Radiation Diagnostics

Russian Federation, Ryazan

N. V. Korotkova

Ryazan State Medical University

Email: fnv8@yandex.ru

Ph.D. (Med.), Associate Professor, Department of Biological Chemistry with Course of Clinical Laboratory Diagnostics, Continuing Professional Education Faculty, Senior Researcher

Russian Federation, Ryazan

References

  1. Patel S., Homaei A., El-Seedi H.R., Akhtar N. Cathepsins: Proteases that are vital for survival but can also be fatal. Biomed Pharmacother. 2018; 105: 526–532; https://doi.org/ 10.1016/j.biopha.2018.05.148.
  2. Wu H., Du Q., Dai Q., Ge J., Cheng X. Cysteine protease cathepsins in atherosclerotic cardiovascular diseases. Atheroscler Thromb. 2018; 25(2): 111–123; https://doi.org/ 10.5551/jat.RV17016.
  3. Упоров М.М. Современное состояние проблемы лечения тромбофлебита поверхностных вен нижних конечностей. Наука молодых (Eruditio Juvenium). 2022; 10(4): 471–490; https://doi.org/10.23888/HMJ2022104471-490.
  4. Zhang X., Luo S., Wang M., Shi G.P. Cysteinyl cathepsins in cardiovascular diseases. Biochim Biophys Acta Proteins Proteom. 2020; 1868(4): 140360; https://doi.org/10.1016/ j.bbapap.2020.140360.
  5. Scarcella M., d'Angelo D., Ciampa M., Tafuri S., Avallone L. Pavone L.M., De Pasquale V. The key role of lysosomal protease cathepsins in viral infections. Int. J. Mol. Sci. 2022; 23(16): 9089; https://doi.org/10.3390/ijms23169089.
  6. Serrano-Puebla A., Boya P. Targeting the lysosome lysosomal membrane permeabilization in cell death: new evidence and implications for health and disease. Annals of the New York academy of sciences. 2016; 1371(1): 30–44; https://doi.org/10.1111/nyas.12966.
  7. Anes E., Pires D., Mandal M., Azevedo-Pereira J.M. Spatial localization of cathepsins: Implications in immune activation and resolution during infections. Front Immunol. 2022; 13: 955407; https://doi.org/10.3389/fimmu.2022.955407.
  8. Sendler M., Maertin S., John D., Persike M., Weiss F.U., Krüger B., Wartmann T., Wagh P., Halangk W., Schaschke N., Mayerle J., Lerch M.M. Cathepsin B activity initiates apoptosis via digestive protease activation in pancreatic acinar cells and experimental pancreatitis. Biol Chem. 2016; 291(28): 14717–31; https://doi.org/10.1074/jbc.M116.718999.
  9. Mehra S., Kumar M., Manchanda M., Singh R., Thakur B., Rani N., Arava S., Narang R., Arya D.S., Chauhan S.S. Clinical significance of cathepsin L and cathepsin B in dilated cardiomyopathy. Mol Cell Biochem. 2017; 428(1-2): 139–147; https://doi.org/10.1007/s11010-016-2924-6.
  10. Li Y., Yang D., Sun B., Zhang X., Li F., Liu Z., Zheng Y. Discovery of crucial cytokines associated with abdominal aortic aneurysm formation by protein array analysis. Exp. Biol. Med. (Maywood). 2019; 244(18): 1648–1657; https://doi.org/10.1177/ 1535370219885101.
  11. Stepien KL., Bajdak-Rusinek K., Fus-Kujawa A., Kuczmik W., Gawron K. Role of extracellular matrix and inflammation in abdominal aortic aneurysm. Int J Mol Sci. 2022; 23(19): 11078; https://doi.org/10.3390/ijms231911078.
  12. Kalinin R.E., Pshennikov A.S., Abalenihina Ju.V., Suchkov I.A., Mzhavanadze N.D., Isakov S.A. Katepsiny kak vozmozhnyj sposob adaptacii sosudistoj stenki k okislitel'nomu stressu v uslovijah ishemii i reperfuzii. Medicinskij vestnik severnogo Kavkaza. 2017; 12(2): 191–194; https://doi.org/10.14300/mnnc.2017.12053.
  13. Weiss-Sadan T., Gotsman I., Blum G. Cysteine proteases in atherosclerosis. FEBS J. 2017; 284(10): 1455–1472; https://doi.org/10.1111/febs.14043.
  14. Kalinin R.E., Suchkov I.A., Shanaev I.N., Agapov A.B., Hashumov R.M. Klinicheskoe nabljudenie pacienta s posttravmaticheskoj arterio-venoznoj fistuloj bedrennyh sosudov v nizhnej treti bedra: osobennosti patogeneza i slozhnosti vedenija. Rossijskij mediko-biologicheskij vestnik im. akademika I.P. Pavlova. 2022; 30(3): 375–386; https://doi.org/10.17816/PAVLOVJ102594.
  15. Becker B., Jacob M., Leipert S., et al. Degradation of the endothelial glycocalyx in clinical settings: searching for the sheddases. Br. J. Clin. Pharmacol. 2015; 80(3): 389–402; https://doi.org/10.1111/bcp.12629.
  16. Barrett A.J., Kirschke H. Cathepsin B, Cathepsin H, Cathepsin L. Methods in Enzymol. 1981; 80: 535–561; https://doi.org/ 10.1016/S0076-6879(81)80043-2.
  17. Wiederanders B., Kaulmann G., Schilling K., et al. Functions of propeptide parts in cysteine proteases. Current Protein and Peptide Science. 2003; 4: 309–326. doi: 10.2174/1389203033487081.
  18. Maksimenko A.V. Molekuljarnye aspekty transljacionnoj kardiologii v issledovanijah sosudistoj stenki. Kardiologija. 2017; 57(7): 66–79; https://doi.org/10.18087/ cardio.2017.7.10008.
  19. Voropaeva A.A., Golubinskaja P.A., Sadovoj M.A., Rusova T.V. Sravnenie aktivnosti katepsinov vo vnutrennih organah krys, kak istochnikah lizosomal'nyh proteaz dlja issledovanija kletok hrjashhevoj i kostnoj tkanej. Sovremennye problemy nauki i obrazovanija. 2016; 5: 25343; https://science-education.ru/ru/article/view?id=25343.

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2. Drawing. Activity of cathepsin B (a), cathepsin L (b), cathepsin H (c), in varicose vein homogenate; * - statistically significant differences from the control group (p<0.05)

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