Development of quality indicators of trichoderma atrobrunneum culture fluid extract vkpm f-1434
- Authors: Gneusheva I.A.1, Lushnikov A.V.2
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Affiliations:
- Orel State Agrarian University named after N.V. Parakhin
- Orel Regional Center for Agricultural Biotechnology, N.V. Parakhin Orel State Agrarian University
- Issue: Vol 26, No 12 (2023)
- Pages: 40-48
- Section: Pharmaceutical chemistry
- URL: https://journals.eco-vector.com/1560-9596/article/view/625485
- DOI: https://doi.org/10.29296/25877313-2023-12-05
- ID: 625485
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Abstract
Relevance. It is relevant to solve the problems of quality control of medicinal substances, drugs, preparations by conducting pharmaceutical analysis.
The purpose of the study – determination of physico-chemical and biological properties of Trichoderma atrobrunneum VKPM F-1434 culture fluid extract. Development of a method for determining the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 in bioproducts.
Material and methods. The strain Trichoderma atrobrunneum VKPM F-1434 synthesizing low molecular weight biologically active compounds with bacteriostatic effect was used in the work. The extract was obtained by single-stage mixing and settling extraction with ethyl acetate from the culture liquid of T. atrobrunneum VKPM F-1434 after 5 days of deep cultivation at a temperature of 28 ° C, the seed material was the vegetative mycelium of the producer in the late exponential growth phase. The mass concentration of
T. atrobrunneum VKPM F-1434 culture fluid extract was determined in a healing liniment for wound healing.
Results. Based on the study of physico-chemical and biological properties of culture fluid extract T. atrobrunneum VKPM F-1434, its individual characteristics have been established. The method "Determination of the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 in the finished bioproduct" has been developed. Scope of the method: determination of the extract’s mass concentration at the stages of the technological process of preparation, as well as in ready-made dosage forms, therapeutic and preventive orientation of zootechnical and veterinary purposes. Characteristic of the method: the range of determined values – mass concentrtion (ω): 1 – 10%; repeatability: CV = 1.69%; reproducibility CV1 = 1.68%; CV2 = 1.78%; Fr = 1.09; Ft = 3.44; correctness:
R = 100.1%.
Conclusions. Physico-chemical and biological properties of culture fluid extract T. atrobrunneum VKPM F-1434 can be used to establish its authenticity and quality assessment. The method of determining the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 can be considered suitable for a reliable assessment of the composition of the finished bioproduct.
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About the authors
I. A. Gneusheva
Orel State Agrarian University named after N.V. Parakhin
Author for correspondence.
Email: obc1-2010@mail.ru
Candidate of Sciences in Technology, Associate Professor of the Department of Biotechnology
Russian Federation, OrelA. V. Lushnikov
Orel Regional Center for Agricultural Biotechnology, N.V. Parakhin Orel State Agrarian University
Email: alex_de-vil@mail.ru
Chief Specialist
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