Development of quality indicators of trichoderma atrobrunneum culture fluid extract vkpm f-1434

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Relevance. It is relevant to solve the problems of quality control of medicinal substances, drugs, preparations by conducting pharmaceutical analysis.

The purpose of the study – determination of physico-chemical and biological properties of Trichoderma atrobrunneum VKPM F-1434 culture fluid extract. Development of a method for determining the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 in bioproducts.

Material and methods. The strain Trichoderma atrobrunneum VKPM F-1434 synthesizing low molecular weight biologically active compounds with bacteriostatic effect was used in the work. The extract was obtained by single-stage mixing and settling extraction with ethyl acetate from the culture liquid of T. atrobrunneum VKPM F-1434 after 5 days of deep cultivation at a temperature of 28 ° C, the seed material was the vegetative mycelium of the producer in the late exponential growth phase. The mass concentration of
T. atrobrunneum VKPM F-1434 culture fluid extract was determined in a healing liniment for wound healing.

Results. Based on the study of physico-chemical and biological properties of culture fluid extract T. atrobrunneum VKPM F-1434, its individual characteristics have been established. The method "Determination of the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 in the finished bioproduct" has been developed. Scope of the method: determination of the extract’s mass concentration at the stages of the technological process of preparation, as well as in ready-made dosage forms, therapeutic and preventive orientation of zootechnical and veterinary purposes. Characteristic of the method: the range of determined values – mass concentrtion (ω): 1 – 10%; repeatability: CV = 1.69%; reproducibility CV1 = 1.68%; CV2 = 1.78%; Fr = 1.09; Ft = 3.44; correctness:
R = 100.1%.

Conclusions. Physico-chemical and biological properties of culture fluid extract T. atrobrunneum VKPM F-1434 can be used to establish its authenticity and quality assessment. The method of determining the mass concentration of culture fluid extract T. atrobrunneum VKPM F-1434 can be considered suitable for a reliable assessment of the composition of the finished bioproduct.

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作者简介

I. Gneusheva

Orel State Agrarian University named after N.V. Parakhin

编辑信件的主要联系方式.
Email: obc1-2010@mail.ru

Candidate of Sciences in Technology, Associate Professor of the Department of Biotechnology

俄罗斯联邦, Orel

A. Lushnikov

Orel Regional Center for Agricultural Biotechnology, N.V. Parakhin Orel State Agrarian University

Email: alex_de-vil@mail.ru

Chief Specialist

俄罗斯联邦, Orel

参考

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2. Rice. 1. Spectrum (a) and the second derivative of the spectrum (b) of the extract of the cultural liquid of T. atrobrunneum VKPM F-1434

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3. Rice. 1. Spectrum (a) and the second derivative of the spectrum (b) of the extract of the cultural liquid of T. atrobrunneum VKPM F-1434

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4. Rice. 2. Dependence of optical density on the mass fraction of the extract of the cultural liquid of T. atrobrunneum VKPM F-1434: a – general graph on a logarithmic scale OX; b – linear range

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5. Rice. 2. Dependence of optical density on the mass fraction of the extract of the cultural liquid of T. atrobrunneum VKPM F-1434: a – general graph on a logarithmic scale OX; b – linear range

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