Vol 70, No 8 (2021)

Introduction. The new promising sulfur-containing phenolic antioxidant dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide has been developed at the Department of Chemistry, Novosibirsk State Pedagogical University. The antioxidant activity of this compound has been shown in studies using various models. It has also been found to be able to have hepatoprotective and anti-inflammatory activities, hemorheological, antiaggregatory, antiplatelet, and pronounced protective effects in cerebral ischemia. Objective: to develop a bioanalytical procedure for estimating the content of dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide in biological material for further pharmacokinetic studies Material and methods. Differential pulse voltammetry was chosen to develop this procedure. The determination was carried out on a working mercury-film (amalgam) electrode using a silver chloride electrode as a reference electrode. The background electrolyte was a mixture of 95% ethanol and 2M sodium hydroxide solution (4:6). Results. The electrochemical behavior of dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide was investigated and conditions for its rat serum quantification were selected. The developed procedure was validated. This procedure could establish the pharmacokinetic parameters of dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide in the rats after its single intragastric administration at a dose of 500 mg/ kg. Conclusion. The developed procedure for the voltammetric determination of rat serum dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide can be used to identify its pharmacokinetic parameters during preclinical studies.

Introduction. As of now, Pharmacopeia Article (PA) 2.5.0009.15 «Setwell (Valeriana officinalis) rhizomes and roots» in the 14th Edition of the Russian Federation's Pharmacopeia does not specify the list of closely related species that can be harvested and used, as is laid down for other types of plant materials (for example, PA.2.5.0106.18 «Brier (Rosa) hips»). The morphological convergence of related species and simultaneously the description of the multitude of different intraspecific forms complicate the differentiation of the species of the Officinales series; therefore, microscopic analysis is used to determine their identity. The literature lacks information on the use of the luminescence analysis described in the Pharmacopoeia to assess the identity of the rhizomes and roots of various setwell species, which determines the relevance of such investigations. Objective: to investigate the morphological, anatomical and diagnostic features of Volga setwell (Valeriana wolgensis Kazak) raw material by luminescence microscopy. Material and methods. The investigation object was the dried aboveground and underground parts of Volga serwell growing in the Voronezh Region. The morphological, anatomical, and diagnostic signs of the raw materials were macroscopically and microscopically examined by luminescence microscopy using a Micromed-3 Lum luminescent microscope. Results. Luminescence microscopy was used to determine and visualize the morphological signs of the aboveground parts of Volga setwell and the anatomical markers of its underground parts. The investigators established the nature of the structure and luminescence of trichomes, conductive elements, and parenchyma of the Volga setwell leaf blade. They revealed the feature of the luminescence of the hypodermis and ground parenchyma of the setwell root, which makes it possible to use this feature as an identification sign when establishing the identity of this raw material. Conclusion. The findings can be used to compile «keys» for the identification of closely related plant species by anatomical features. Investigations will contribute to the further comprehensive study of setwell species belonging to the Officinales Grub., which account for 8 to 11 species according to different data.

Introduction. Love-in-a-mist (Nigellasativa L.) is a representative of the ranunculaceous family (Ranunculaceae). Nigellasativa L. is insufficiently studied as compared with other Nigella L. species, including wild fennel (Nigella damascena); however, it is a promising type of medicinal plant material, for which hepatoprotective, hypoglycemic, antioxidant, antibacterial, anthelmintic, and general healthimproving actions have been described. In addition to the use of the main raw material, such as the seeds of Nigella sativa L., of interest are its aboveground parts (herb) that have still been little studied and have not had a wide application in scientific medicine. Therefore, there is a need for pharmacognostic studies, followed by the development of regulatory documents confirming the identity and quality of this medicinal plant for promising raw materials. Objective: to develop a procedure for quantification determination of the amount of flavonoids in the Nigella sativa L. herb. Material and methods. The investigation object was the Nigella sativa L. herb harvested in July 2021 in the Ulyanovsk Region and in the Botanical Garden of the Samara Medical University. Direct and differential spectrophotometries were used to develop the procedure. Results. The optimal conditions were determined for quantification of the amount of flavonoids in the Nigella sativa L. herb; these were an extractant of 70% ethanol; a raw material-extractant ratio of 1:30; an extraction time of 45 min, and a raw material grinding size of 2 mm. Validation studies showed the validity of the developed procedure. Conclusion. The procedure was developed for quantification of the amount of flavonoids in the Nigella sativa L. herb, by using differential spectrophotometry at an analytical wavelength of 412 nm, calculated with reference to the standard sample (SS) - rutin. The content of the amount of flavonoids in the herb of the Nigella sativa L. cultivated in the Ulyanovsk and Samara Regions ranges 1.17 to 1.71%. The error of single determination at 95% confidence probability is ±4.17%.

Introduction. The socioeconomic burden of rheumatic diseases (RDs) is an important public health problem due to the annually growing epidemiological indicators and high patient disability rates. The use of expensive biological agents (BAs) in pharmacotherapy could achieve the major goal of treatment, such as remission and better quality of life in a number of cases. In the context of budget deficits, it is of great importance to determine the optimal hospital demand forecast for BAs, taking into account the influence of various factors on drug consumption, which will be able to increase the availability of targeted therapy for all patients in need. Objective: to determine the optimal hospital demand for BAs for the treatment of patients with RDs, by using the multifactorial mathematical modeling method. Material and methods. The materials were expert review questionnaires, 1181 case history sheets for patients diagnosed with rheumatoid arthritis, ankylosing spondylitis, and psoriatic arthritis. Expert assessments, content analysis, correlation regression analysis, and multifactorial mathematical modeling were used. Results. Multifactorial mathematical models were developed and the 2020-2023 hospital demand forecast for BAs (rituximab, certolizumabapegol, golimumab, tocilizumab, and abatacept) was estimated. Conclusion. The short-term demand forecast calculated using multifactorial mathematical modeling was reliable and can be used when creating the optimal purchase order for BAs (rituximab, certolizumabapegol, golimumab, and tocilizumab).