Vol 7, No 6 (2019)

The morphological features of the skin of rats under the stress exposure, such as burn injury and against the background of the correction by the drugs of the peptide structure, have been studied.The aim of the study was to investigate the regulatory effect of the neuropeptide drugs Semax (Met-Glu-His-Phe-Pro-Gly-Pro) and Selank (Thr-Lys-Pro-Arg-Pro-Gly-Pro) under the conditions of thermal burn exposure.Materials and methods. The object of the study was integumentary tissues (skin, subcutaneous tissue) of laboratory non-linear male rats (n = 36) excised from the thermal injury zone. A thermal burn of the skin had been caused by the application of a copper object in the interscapular in the interscapular dorsal area. Starting from the first day after the injury, neuropeptide drugs Semax (Met-Glu-His-Phe-ProGly-Pro) and Selank (Thr-Lys-Pro-Arg-Pro-Gly-Pro) had been administered intraperitoneally at the doses of 100 μg/kg daily during the entire period of the experiment. To assess the condition of the skin tissues, histological sections 5-6 μm thick were prepareв, stained afterwards with hematoxylin and eosin. The consistency of the systemic effect of the neuropeptide drugs was estimated by a change in some indicators of the immune system.Results. The thermal exposure led to the development of significant degenerative and dystrophic changes in the skin. The recovery of the burn wounds on rats’ skins proceeded according to the type of the delayed partial reparative regeneration, accompanied by destructive phenomena and the formation of the scar tissue.Under the influence of the drugs based on the regulatory peptides of Semax (Met-Glu-His-Phe-Pro-Gly-Pro) and Selank (Thr-Lys-Pro-Arg-ProGly-Pro), the recovery of burn wounds in rats proceeded more intensively, compared with the animals from the group without any correction. This fact was confirmed by the earlier signs of the beginning of the reparative skin regeneration: the restriction of destructive processes within the epidermis and dermis, the absence of purulent-necrotic complications, the initial phases of granulation and epithelization, an early scab rejection and a partial closure of the defect.Conclusion. The use of the neuropeptide drugs Semax and Selank as systemic remedies for the correction of wound skin defects in experimental animals proves their polypotent effectiveness, expands the therapeutic possibilities and opens up new prospects for their use.

Nowadays, the search for new effective and safe medicines for the treatment of acid-dependent gastrointestinal diseases remains an urgent problem of modern pharmacology.The aim of this study was an experimentally study of the anti-ulcer activity of 2-phenyl-9-diethylaminoethylimidazo[1,2-a]benzimidazole dinitrate on the model of damage to the gastric mucosa caused by the administration of 80% ethanol and prednisolone combination (20 mg/kg).Materials and methods. To simulate the damage to the gastric mucosa, the experimental animals (white male Wistar rats) were administrated with prednisone at the rate of 20 mg/kg and 80% ethyl alcohol at the dose of 0.6 ml/100 g of the animal body weight. Prednisolone was dissolved in 80% alcohol. Antisecretory antiulcer agents actively used in clinical practice, were selected as reference drugs: ranitidine (30 mg/kg, 10 mg/kg and 3 mg/kg) and omeprazole (3 mg/kg, 1 mg/kg and 0.3 mg/kg). The studied compound was used at the doses of 30 mg/kg, 10 mg/kg and 3 mg/kg. All the substances under study were administered intragastrically with the use of an atraumatic probe. Results. It has been established that the benzimidazole derivative in the studied doses contributes to a dose-dependent reliable reduction in the area and depth of ulcerative lesions of the gastric mucosae relative to the control and reference drugs (ranitidine and omeprazole). In addition, in the maximum studied dose (30 mg/kg), the proportion of the animals with ulcerative lesions significantly decreases by more than 2 times. The calculated ED50 values for the benzimidazole derivative and ranitidine were 5.09 mg/kg and 38.23 mg/kg, respectively.Conclusion. The obtained experimental data indicate that the benzimidazole derivative has a pronounced dose-dependent antiulcer effect on the model of ethanol-prednisolone erosive-ulcerous defects of the rats’ gastric mucosae, which is superior to the effects of the reference preparations. It makes its further study promising.

The aim of the study was to investigate the prevalence of acute viral hepatitises A, B and C in the Volgograd region; the range and identification of the most frequently prescribed pharmacological groups to be used in hospital environment; and analyzing the price dynamics of medicines. These factors will make it possible to assess the state of the medicine provision for the patients with this disease in hospital environment. Materials and methods. In the study, the following methods have been used: comparison, a method of grouping indicators and a structurallogical method. The materials were the hepatological department patients’ treatment sheets (Volgograd Regional Clinical Infectious Diseases Hospital N1).Results. Acute hepatitis A is the most common (46.9%), acute hepatitis B occupies the second position (27.4%), and acute hepatitis C - the third one (25.7%), where in 46.2%, 23.5% and 14.7%, respectively, are accounted for by icteric viral hepatitis. Hepatoprotectors, symptomatic medications and rehydration and detoxification medicinal preparations are prescribed for all forms and degrees of the disease severity.Conclusion. The study has revealed a decreased morbidity of acute viral hepatitises A, B and an increased morbidity of acute viral hepatitis C in the Volgograd region. Among all the types of hepatitises, the prevailing one is the icteric form of moderate severity. The range of medicinal preparations prescribed for the treatment of acute viral hepatitises has been studied. Most often, doctors prescribe hepatoprotectors, rehydration and detoxification medicinal preparations. The study of price dynamics, showed a predominant increase in hepatoprotectors and a decrease in medicines for rehydration and detoxification. The results obtained indicate a tendency towards the improvement of drug provision in the Volgograd Region, for the patients with acute viral hepatitises. Besides, the results of the study give an opportunity to consider the ways of its further optimization.