Vol 14, No 3 (2014)

The information presented is about the life and creative activity of the researcher of Experimental Medicine Institute, who is a prominent Russian pathophysiologist Peter Nikolayevich Veselkin, that includes the main research areas of his activity, social environment, personal features, and life interests. Work has been prepared for the monograph «Scientists of Saint-Petersburg Medical Academy of Postgraduate Study» and is published in the journal format for the first time.

The calbindin immunocytochemistry-based fluorescent technique is proposed for revealing the dendrites of the Purkinje cells in the human cerebellum. By improving the sensitivity of the immunocytochemical reaction and application of embedding medium with a high refractive index, fluorescence level was reached sufficient for the high resolution laser confocal microscopy and the informative three-dimensional reconstructions. Despite the use of the fixed material and the paraffin sections the presented protocol provides high-quality visualization of the structures studied.

Psychogenic stress in rabbits caused inhibition of contractile activity (CA) in antral and pyloric parts of the stomach. This reaction is preserved after blockade of a2-adrenergic receptors, β1/β2-adrenergic receptors, M or N-cholinergic receptors. We conclude that inhibition of gastric motility under stress is substantially «non-adrenergic non-cholinergic» and only in the initial phase of the reaction it appears to be «α-adrenergic». The latter mechanism also determines the initial transient inhibition of CA in duodenum. The subsequent strengthening of CA in the proximal duodenum is preserved after blockade of M or N-cholinergic receptors and is the result of direct exciting action of the endocrine stress factor on the smooth muscle of the gut. Strengthening of the CA in the distal duodenum is abolished by blockade of M or N-cholinergic receptors, as well as β1/β2-adrenergic receptors and is a consequence of the endocrine action of catecholamines on stimulating β-adrenergic receptors of enteric cholinergic neurons. The same mechanism is responsible for the initial increase of CA in jejunum, which is replaced by its inhibition, which is «non-adrenergic non-cholinergic». Inhibition of CA in the initial phase of the stress-induced response of the ileum, cecum and colon is due to «α-adrenergic» mechanism, and subsequent period of the CA inhibition is the result of the «non-adrenergic non-cholinergic» mechanism. Strengthening of the CA in the initial part of the distal colon is abolished by the blockade of M or N-cholinergic receptors, and is caused by the centrogenic stimulation of preganglionic neurons by endocrine stress factor with subsequent activation of effector cholinergic neurons of the enteric nervous system.

The aim of this study was to investigate clinical and pathogenetic features of myocardial infarction, medical correction of this pathology using proteolytic enzymes. The use of proteolytic enzymes (drug Phlogenzym) in the early stages contributes to correction and inhibition of structural-functional restructuring of the heart. Concentric remodeling is the predominant type of structural transformation of the myocardium of the left ventricle. Cardioprotective effect of systemic enzyme therapy is most pronounced in the early post-infarction period (up to 3 months) that is combined with a higher quality of life for patients.