Vol 13, No 2 (2013)

The role of dispersive medium (physiological solution, light mineral oil, and ester-modified olive oil) was estimated in antitumor effect of ferromagnetic implant in SHR mice with intramuscular LIO-1 lymphosarcoma. Regardless of media used for implantation of barium hexaferrite, animals with LIO-1 showed mild antitumor effect and increase in life-span of SHR mice.

Therapy resistant bronchial asthma is the most difficult for treatment and has the most unpredictable course. In spite of the fact that multiple pathogenetic mechanisms of asthma had been studied, mechanisms of therapy resistant asthma still remain unclear. Moreover, this problem seems to be not well investigated. Up to present time there is no exact definition of the therapy resistant bronchial asthma phenotype. Also, the term therapy resistant asthma is not widely used. The aim of study was to evaluate the role of pharmacogenetic factors in glucocorticosteroid and beta2-agonists treatment in therapy resistant bronchial asthma. Also the investigation was aimed on working out of the prognostic criteria for treatment resistance taking into attention of genetic markers. In present work the results of investigation are discussed. These results gave possibility to determine the phenotype of therapy resistant bronchial asthma taking into attention pathogenetic and clinical heterogeneity. The results of study also gave possibility to find out the predictors of the disease and to work out the diagnostic criteria based on clinical peculiarities and genetic determinants.

117 BA pregnant women and their kids were followed up and treated within 5 to 10 years. Kids' health status was estimated up to end of follow up. Аllergic pathology level within BA mothers' born kids was significantly higher, than in whole population (52,9% & 25%). Main risk factors of allergic pathology appearance within first 7 years of life are: allergic diseases in both parents (p=0,08), infantile phenotype of BA in kids' mother, (p<0,05), weak genotype of IL-4 (-590 C/T), IL-4R (Q576R), TNF-α (-308G>A) genes. Molecular-genetic study of atopic causal genes could be recommended for allergic risk screening in kids in order to perform further diagnostics and prophylaxis.

An immunohistochemical study of biopsy samples obtained from 7 patients with chronic pancreatitis were performed using reactions to neurofilaments (NF), synaptophysin (Syn), chromogranin (CgA), myelin basic protein (MBP), and protein gene product 9.5 (PGP 9.5). Results obtained demonstrated high selectivity for revealing the endocrine cells of the islets of Langerhans for three markers: Syn, CgA, and PGP 9.5. In all the cases under investigation, the head of pancreas exhibits significant dystrophic and degenerative changes in neurons of intramural ganglia, nervous stems and bundles, demyelination of nervous fibers, destructive changes in epi-, peri-, and endoneurial sheaths, decrease or total loss of Syn-immunopositive efferent terminals around blood vessels and pancreatic ducts, denervation of glandular tissue of the exocrine and endocrine parts. Besides impairment of innervation, dystrophic changes and reorganization of the islets of Langerhans accompanied by migration and dedifferentiation of various kinds of endocrinocytes were found. In some cases, reaction to PGP 9.5 revealed an increased number of immunopositive nervous bundles, nervous fibers and their terminal branches in the hypertrophic connective tissue, around vessels, and inside or around inflammation infiltrates. It is suggested that PGP 9.5-immunopositive fibers are (nociceptive) afferent receptor apparatuses perceiving pain. The found inflammation infiltrates with a lot of plasmatic cells selectively clustered around the nervous ganglia, nervous trunks and bundles as well as disintegration of myelin sheaths revealed using MBP reaction in pancreatic nerves suggest possible development of autoimmune processes.