Vol 51, No 4 (2002)

In this view, the up-to-date information on proteins including selen is presented. Here is considered the conduct of selen proteins of glutationperoxidases family during pregnancy and its complications.

The paper presents data on the possibilities of determining urogenital mycoplasmas (Mycoplasma hominis and Ureaplasma urealyticum) using some variants of culture and PCR methods. The scheme of cultural studies in full is given, with the help of which high specificity and sensitivity indicators are achieved. The proposed research algorithm for detecting Mycoplasma hominis and Ureaplasma urealyticum, based on the use of culture and PCR methods as complementary.

This article is devoted to optimization of dispensary observation at the eshlia terms of gestation of women suffer from sporadic miscaniages in anamnesis as well as tlie definition of prognostic meaning of the ultmsonic doppleromet-ry criterias, the enzyme activity of lisosomal of blood plasma and tlie cytochemical mte (DCR) for tlie segmentnuclear leucocytes at the pregnant women witli pathological obstetl'ic anamnesis.

13 7p1-egnant women were tested, 98 o f them at the earlier terms o f gest-ation from 7 to 14 weelis of t-heir pregnancy. The received data show that metabolic conection and normalization of microbiocenosis of vagina and cavix channel in tlie first trimester of pregnancy is pathogen validity, they promot-e tlie nmwaf pregnancy of the next terms of gestation and decrease tlie frequency of repeat-ed misriages

The complex investigation of the perinatal pathology at acute and c/1,1-onic psyclioemotliional stress dunng gestation has been perf 01 wed. Jt was sliown (by tlie metliod of the retrospective analyses) the inaease in the relat-ive 1-isf of tlie premature delivery (1,4-8,0), severe and mild gestosis (3,0-10,0), placental pathology (2,2-3,3 ), anomalies of delivery (2,0-7,0 ), obstetl′ical Blending (2,0- 2,8 ), late delivery (2,0-7,0) dunng the acute stress. The relationship between tlie pel′inatal patology and gestation term was shown dunng the acute stress. Dm'ing- i-lie clironic stress tlie frequency and seventy of pennatal patliology is dependent upon the type of psyclioemotional reaction on the st-ress. The seventy of patliofog-y is more prominent at decompensated type of psychological reaction t-lian at subcompensated type. Tlie newbom infants of motlias witli psyclioemotional stress recuired long rehabilitation in 39,2-50,0% cases.

In work on the basis of carried out morphological and roentgenological investigations the anatomic substantiation of intraosseous injections of antibiotics at treatment of endometritis and purulent adnexitis is given. The positive results of therapy with application of intraosseous introduction of medicinal preparations 25 women with aп endometritis after operation are resulted is cesarean sections and 30 women with purulent adnexitis. The indications to application of the given way of introduction of medicinal preparations are proved.

Goal of the research: optimization of the diagnosis of chronic endometritis.

Methodology: perspective research.

Institution: Department of obstetrics and gynecology of the Kursk medical University.

Material of the research: 67 patients suffering benign diseases of endometrium and myometrium connected with it. 59 patients with the diseases of the uterus of the same пaтe without chronic endometritis.

Methods of the research: clinico-laboratory, ultrasonography, hysteroscopic, histologic, cytologic, microbiologic examination of the endometrium, PSR, radioimmunological method to determine progesterone rate in the blood plasma.

Results of the research: the endoscopice variants of chronic endometritis are extreted: hyperplastic and hypoplastic (and also its forms - focal and diffusive). They are necessary stages achieving reliable diagnosis of the inflammatory process of endometrium a differential method of probing of endometrium depending on a variant and damage rate of chronic endometritis is worked out.

Conclusion: the complex method of the diagnosis chronic endometritis including hysteroscopy with visual biopsy and cytologic-histologic examination of endometrium increases the quality of diagnosis by 64,4% in comparison with the traditional curettement of the endometrium and considerably decreases the number of posttraumatic and inflammatory complications.

In order to estimate the colposcopic manifestations of cervical oncotropic human papilloma-virus (HPV)-infection, a total of 693 patients were examimed. Among them, there were 298 patients with benign tumors pathology, 57 patients with 1-3 Grade cervical dysplasia of mucosa, 50 patients with uterine cervix cancer, 288 healthy women.

All patients underwent bimanual examination, taking of cervical smears for cytological examination and uterine cervix colposcopy. Diagnosis for HPV16/18 infection was made by the method of polimerase chain reaction.

А large variety in colposcopic manifestations of HPV-infection was found, namely: areas of atypical vessels, leukoplakia sites,fields of atypical epithelium, iodine-negative sites. It was related to the influence of oncogenic types of HPV infection. In these patients,fields of atypical epithelium, atypical vessels, iodinenegative areas were observed 1.2, 2.5, 10.5 times more frequently, respectively.

It was found that all varieties of papillomas occurred among patients with pathology, whereas flat condylomas presenting the most difficulties of or diagnosis prevailed in patients with cervical neoplasms and uterine cervix cancer The most pronounced colposcopic evidences of uterine cervix epithelium malignancy were observed in patients with virus-positive uterine cervix cancer.

In experiments with 60 segments of arteries and 46 segments of the umbilical cord vein of 24 newborns and 35 strips of myometrium of 12 pregnant women, the effect of 100-fold dilutions of cord blood serum (SPK-1: 100) on the contractile effects of histamine (0.01, 0.1 and 1 ϻg l ml). SPK-1: - 100 increased the vasoconstrictor and uterostimulating effects of histamine.

H₁-histaminosensitizing activity of SPK-1: 100 and the efficiency of activation of H₁-histamine receptors of myocytes of the arteries and umbilical cord veins are increased in the presence of obstetric complications. A conclusion was made about the content of endogenous sensitizer H₁-histamine receptors (ESGR) in the fetal blood, the level of which increases in the presence of obstetric complications, as well as the possible role of histamine and ESGR in the regulation of fetoplacental blood circulation in conditions of normal and pathological conditions.

HPV 16 and 18 are known to be the main cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The terms of HPV persistence in the host and, coordinately, the risk of cervical neoplasia development and progression are determined in much extent by virus activity. The purpose of this investigation was the detection of HPV DNA presence in cervical epithelium as well as confirmation of its activity by means of immunocytochemistry and reverse transcriptase polymerase chain reaction. The level of HPV inf ection by oncogenic and nononcogenic types in 181 women with different cervical pathology was 55,8%. The active stage of HPV infection was confirmed in 27,5% of HPV-inf ected women mainly with low grades of CIN. The proof of reproductive general HPV infection was more informative with RT PCR just as for HPV 16 and 18 immunocytochemistry and RT PCR completed each anothe.

The study of a deliberately induced miscarriage is of extreme interest from a wide variety of points of view: first of all, from a cultural and historical point of view, then from a socio-legal and, finally, from a medical one. For what reasons and when a woman first resorted to artificial termination of pregnancy, it is not possible to establish precisely.