Dynamics of proliferation of L. reuteri and L. helveticus

Effects of the quantity of inoculate added and changes of the active acidity on the proliferation dynamics of L. reuteri and L. helveticus in the process of mutual cultivation have been studied. At the simultaneous introduction of the inoculate of the microorganisms studied in the ratios 1:10 and 1:50 maximum accumulation of cells has been noted after 6 h cultivation -8 -109and 6,5 • 109accordingly. At the stage by stage addition of the culture L. reuteri (6 %) and L. helveticus (1,2 and 3 %) the most intensive growth of L. reuteri has been achieved after 6 h. when 2 % of L. helveticus were applied. At mutual cultivation of L. reuteri and L. helveticus for 6 h. the largest quantity of the cells L. reuteri is being achieved at the pH 5,8±0,1 and at the quantity of the inoculate L. helveticus added 2 %. For L. helveticus these figures are 6,2±0,1 and 2 and 3 % correspondingly.

L. helveticus, L. reuteri, bacterial concentrate, L. helveticus, L. reuteri, mutual cultivation, inoculate, active acidity