Vol 24, No 8 (2021)

Relevance. The potency of raw materials, intermediate products and the finished preparation of human anti-D immunoglobulin must be determined in international units. The application of the international standard for anti-D immunoglobulin in the Russian Federation has difficulties associated with the duration of delivery and the need to comply with increased requirements for the conditions of transportation of biological thermolabile substances. In this regard, there is a need for the development of a domestic standard sample for the content of anti-D antibodies and its certification in international units. An integral step in obtaining this standard is the creation of a raw material base, taking into account the requirements for the content of anti-D antibodies in the immune blood plasma of donors. The aim of the study. To develop an algorithm for the selection of plasma donors for the production of a domestic standard sample for the anti-D antibodies content. Material and methods. The data of the information system of transfusiology for the period from january 2015 to december 2020 were analyzed about 510 donors with identified anti-erythrocyte antibodies. Immunohematological tests were performed by gel centrifugation using reagents and a set of equipment manufactured by Bio-Rad Laboratories (Switzerland). The concentration of anti-D antibodies in the blood plasma of donors was calculated in relation to the 2nd WHO international standard for anti-D immunoglobulin, NIBSC 01/572 (Great Britain). Results. It is proposed to use blood plasma of persons naturally immunized to the D-antigen as a substance for the production of a standard sample. As a result of the analysis of the requirements for the analytical range of methods for determining the potency of human anti-D immunoglobulin, it was established that the content of anti-D antibodies in the production pool of plasma for obtaining a standard sample should be at least 3.75 IU/ml. The selection of donors begins with the formation from the transfusion information system of list person with Rh-negative affiliation and identified anti-erythrocyte antibodies based on the results of a medical examination. Potential donors of anti-D plasma sent for immunohematological examination for determine the specificity of antierythrocyte antibodies and the content of anti-D antibodies. The presence of specific antibodies to the D-antigen is the basis for including information about the donor in the forming database. Conclusions. The necessity of creating a domestic standard sample for the content of anti-D antibodies has been proved. An algorithm for the formation of a raw material base for the production of a domestic standard sample has been developed. Using the formulated selection principles, a donor base was created, including 22 women (as of December 2020), in whose plasma the anti-D antibodies content is in the range from 0.1 to 28.5 IU/ml.

Relevance. Currently, thyroid diseases have a high social significance, which is determined by a significant increase in their prevalence, large financial costs for diagnosis and treatment. In this regard, the active search for new medicines with a thyroprotective effect continues. Of particular interest are medicinal plants that serve as a source of biologically active substances that affect the main mechanisms of the development of the disease. Such plants include Potentilla alba L., from the roots and rhizomes of which, in the Research institute of medicinal and aromatic plants, a dry extract with a thyrotropic effect was obtained. Due to the fact that one of the mandatory stages of preclinical studies of new pharmacological substances is the study of their effect on the generative function of laboratory animals, it was relevant to study the effect of the dry extract Potentilla alba L. on the ability of female rats to conceive. The aim of the study was to study the effect of the dry extract on the generative function of female experimental rats and their offspring in the pre - and postnatal period of development. Material and Methods. The choice of methods for studying the effect of the dry extract Potentilla alba L. on the generative function of female rats was determined by the requirements set out in the "Guidelines for conducting preclinical studies of medicines". Experiments were performed on male and female Wistar rats weighing 180-200 g (female) and 200-240 g (male). In this study, the test extract was introduced to female rats in the stomach for 15 days at doses of 25 and 125 mg/kg. After the end of the introduction period, the experimental females were mated with intact males. The control animals were intact females and males crossed with each other. The resulting pregnant females were divided into two groups. In the first group of female rats on the 20th day of pregnancy, the number of corpus luteum, positions of implantation, resorptions, live and dead fetuses was calculated, pre-and post-implantation death was calculated; the fetuses were subjected to external examination. From female rats of the other group, offspring were obtained, in which the dynamics of changes in body weight and death before the end of the feeding period (21 days) were taken into account, the rate of maturation of sensory-motor reflexes was determined, and the functional state of the central nervous system was studied according to oriented reactions in the "open field" test. Results. As a result of the experiment, it was found that the introduction of dry extract Potentilla alba L. to female rats in the tested doses before mating with intact males did not affect their ability to conceive. The studied extract did not have a negative effect on the ante - and postnatal development of the offspring of female rats that received the extract before mating with intact males. Conclusion. The dry extract Potentilla alba L. with prolonged introduction into the stomach of female rats in doses exceeding the therapeutic 5 and 15 times, did not affect their generative function and indicators of physical development of the offspring.

For the first time, the results of the total content of some phenolic compounds of 70% water-ethanol extracts of the aboveground part of the Teucrium chamaedrys L. and the assessment of their variability depending on the altitude factor in the natural conditions of Dagestan are presented. The material for the study was collected during the flowering phase in 2013. The content of flavonoids and anthocyanins was determined by spectrophotometric method on SF-56. Total antioxidants - amperometric method. The content of accumulation of phenolic compounds in the aboveground part of T. chamaedrys varies in the range of 2.02-3.06% (the sum of flavonoids); 0.31-0.43 % (the sum of anthocyanins); 4.35-9.64 mg/g (the sum of antioxidants), depending on the population. The influence of the altitude factor on the accumulation of phenolic compounds showed a multi-vector direction, with a significant positive relationship between the altitude above sea level and the content of total antioxidants. In addition, a direct correlation was found between the content of flavonoids and antioxidants, which indicates the key role of flavonoids in the antioxidant status of T. chamaedrys. Statistical methods of analysis made it possible to reveal a greater difference in the variability of the content of phenolic compounds by populations than within populations. Based on the classification matrix, it was possible to divide all samples into separate isolated populations. The results obtained can be used both in the theoretical aspect (revealing the mechanisms of variability of biochemical parameters from abiotic factors), and in the applied meaning.