Development of the nutritient medium for cultivation of the consortium of the probiotic microorganisms

Nutritive media for cultivation of the microorganisms consortium «Ton» were selected with the aim to receive bacterial concentrate for production of the fermented probiotic product «Tonus». The industrial nutritive media with different compositions such as GMK-2, VIS-STAR (MW-50, TW-50), BIOS 2000 have been studied. The media GMK-2 allows receive high level of the living cells of the symbiotic starter after 6 hours cultivation and the media TW-50 - after 10 hours. The number of the living cells of the microorganisms consortium in the medium GMK-2 was 4 • 10 3cfu in 1 cm 3 and in the medium TW-50 - 5 • 10 8 cfu in 1 cm 3. The nutritive media FMK-2 and TW-50 provide reactivation, rapid cells growth and accumulation of the fermenting microflora. That is why in further investigations the media GMK-2, VIS-STAR TW-50 and various components were applied for preparation of nutritive media. The best results as to the biomass received gave the media consisting of the GMK-2 with added peptone and yeasts extract and by the summary quantity of the cells of the microorganisms consortium in the biomass - the media consisting of GMK-2 with addition of peptone and yeasts extract and the media consisting of TW-50 with addition of autolizate and yeasts extract. The largest quantity of the cells was received at cultivation in the nutritive medium with the following composition: GMK-2-12,5 g in 1 dm 3, peptone - 8 g in 1 dm 3, yeasts extract - 10 g in 1 dm 3.

fermented probiotic product «Tonus», bacterial concentrate, nutritive media, symbiotic starter, consortium of microorganisms