Determination of adulteration of the fat phase of milk products with vegetable oils


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Resumo

A method for mass spectrometric identification of the sterol composition of the milk and milk products fat phase which comprises hydrolyzation of sterides in sterols (without preliminary extraction of fat), their chromatographic separation by gas-liquid chromatography and comparing received mass spectra of sterols with mass spectra and retention time of the standard substances is offered.

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Bibliografia

  1. Межгосударственный стандарт ГОСТ 31663-2012 «Масла растительные и жиры животные. Определение методом газовой хроматографии массовой доли метиловых эфиров жирных кислот»
  2. Межгосударственный стандарт ГОСТ 31979-2012 «Молоко и молочные продукты. Метод обнаружения растительных жиров в жировой фазе газожидкостной хроматографией стеринов»
  3. Чмиленко Ф.А. и др. Установление фальсификации молочной продукции методами газовой хроматографии // Методы и объекты химического анализа. 2009. Т. 4. № 1. С. 60-66
  4. Хасанов В.В. и др. Состав жирных кислот и стероидов растительных масел //Химия растительного сырья. 2006. № 3. С. 27-31
  5. Borkovcová I., Janoušková E., Dračková M., Janštová B. and Vorlová L. Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods // Czech J. Food Sci. Vol. 27. 2009. Special Issue. 217-219
  6. Erkki Mäeorga, Peeter Läänistea, Juhan Jõudua, and Uno Mäeorgb. Some important aspects of sterol analysis of vegetable oils // Proc. Estonian Acad. Sci. Chem. 2007. 56,2, 59-66
  7. IDF (2006) IDF 159 ISO 12078 Anhydrous milk fat-Determination of sterol composition by gas liquid chromatography (Reference method). International Dairy Federation, Brussels
  8. IDF (2006b) IDF 200 ISO 18252 Anhydrous milk fat-Determination of sterol composition by gas liquid chromatography (Routine method). International Dairy Federation, Brussels
  9. Du M., Ahn D.U. Simultaneous Analysis of Tocopherols, Cholesterol, and Phytosterols Using Gas Chromatography//Journal of Food Science. Vol. 67. Nr. 5.2002. 1696-1700
  10. Contarini G., Povolo M., Bonfitto E. and Berardi (2002). Quantitative analysis of sterols in dairy products: experiences and remarks. International Dairy Journal. 12. 673-578

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