Sterilization of nutrient medium for production starter cultures

The method of nutrient medium sterilization for the growth of lactic and probiotic microorganisms has been optimized in order to obtain effective starter cultures. The method includes autoclaving at the temperatures range106-108 °C and excessive pressure from 0,3-0,4 atm during 45-60 min. The growth time of microorganisms up to titers of 107-108 cfu/g was 11-12 h. The starter cultures prepared in such a way contain starter microorganisms in classical form at the absence of the foreign microflora. The acidities of the ready for application bacterial starters were 4,5-5,0 units. Possibility to use lower temperatures and pressures during autoclaving in comparison with usually applied parameters can be associated with acidification of starter cultures materials at different stages of their production.

optimization, sterilization, nutrient media, starter cultures